Search results for "Transfer DNA"

showing 2 items of 2 documents

Correlation of plasmid DNA supercoiling and the efficiency of plasmid gene transcription

2007

Transfer DNAPlasmid preparationPlasmidPlasmid dnaChemistryDNA supercoilTechniques of genetic engineeringBioengineeringPromoterGeneral MedicineApplied Microbiology and BiotechnologyMolecular biologyBiotechnologyJournal of Biotechnology
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Plasmid conjugation from Proteobacteria as evidence for the origin of xenologous genes in Cyanobacteria

2014

Comparative genomics have shown that 5% of Synechococcus elongatus PCC 7942 genes are of probable proteobacterial origin. To investigate the role of interphylum conjugation in cyanobacterial gene acquisition, we tested the ability of a set of prototype proteobacterial conjugative plasmids (RP4, pKM101, R388, R64, and F) to transfer DNA from Escherichia coli to S. elongatus. A series of BioBrick-compatible, mobilizable shuttle vectors was developed. These vectors were based on the putative origin of replication of the Synechococcus resident plasmid pANL. Not only broad-host-range plasmids, such as RP4 and R388, but also narrower-host-range plasmids, such as pKM101, all encoding MPFT-type IV …

Transfer DNAGene Transfer HorizontalGenetic Vectorsmacromolecular substancesBiologyOrigin of replicationmedicine.disease_causeCyanobacteriaMicrobiology03 medical and health sciencesPlasmidShuttle vectorSynechococcus elongatus PCC 7942medicineEscherichia coliShuttle vectorMolecular BiologyGeneEscherichia coliSynthetic biology030304 developmental biologyGeneticsSynechococcus0303 health sciences030306 microbiologyElectroporationPlasmid conjugationArticlesHorizontal gene transfer3. Good healthElectroporationType IV secretion systemConjugation GeneticHorizontal gene transferPlasmids
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